|DNA Extraction Kit:||Soil Genomic DNA Rapid Extraction Kit(Spin-column)||DNA Isolation Kit:||Soil Genomic DNA Rapid Isolation Kit(Spin-column)|
|DNA Purification Kit:||SoilGenomic DNA Rapid Purification Kit(Spin-column)|
Soil Genomic DNA Rapid Isolation Kit(Spin-column)
Offered in three sizes (50 preps,100preps, 200preps),DP4031,DP4032&DP4033
Bioteke’s Soil Genomic DNA Rapid Isolation provides a convenient and rapid method for the detection of microorganisms from soil samples. All types of soil samples can be processed with this kit, including common soil samples and difficult soil samples with high humic acid content such as compost and manure. The kit removes all traces of humic acid using the provided Humic Acid Removal Column and the OSR (Organic Substance Removal) Solution. A simple and rapid spin column procedure is then used to further purify the DNA. Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR applications, as all humic acid substances and PCR inhibitors are removed during the isolation.
Bioteke’s Purification Technology
Purification is based on spin column chromatography. The process involves first adding the soil sample, Lysis Buffer G and Lysis Additive A to a provided Bead Tube, and the soil sample is homogenized. The sample is then centrifuged, and the supernatant is transferred to a DNase- free microcentrifuge tube. Binding Buffer I is added, and the lysate is incubated for 5 minutes on ice. This step can then be repeated using the provided OSR (Organic Substance Removal) Solution for soil samples containing high amounts of organic substances as an optional step. The clean lysate is then spun through a Humic Acid Removal Column and the flow through is collected and ethanol is added. Next, the solution is loaded onto a spin-column, which binds only the DNA. The bound DNA is then washed using the provided Buffer SK and Wash Solution A, and the purified DNA is eluted using the Elution Buffer B. The purified total DNA is free of all inhibitors, including humic acid, and can be used in sensitive downstream applications including PCR.
Rapid and convenient method to detect microorganisms in soil samples
Process all soil types
Remove organic substances using the OSR Solution
Remove all humic acid from DNA samples using the Humic Acid Removal Columns
Fast and easy processing using a rapid spin-column format
Isolate high quality total DNA from a variety of microorganisms including bacteria, fungi and algae
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Precautions and Disclaimers
This kit is designed for research purposes only. It is not intended for human or diagnostic use.
Ensure that a suitable lab coat, disposable gloves and protective goggles are worn when working with chemicals.
Customer-Supplied Reagents and Equipment
You must have the following in order to use the Soil DNA Isolation Kit:
► Benchtop microcenrifuge
► 1.7 mL DNase free micro centrifuge tubes
► DNAse-free microcentrifuge tubesFlat bed vortex or bead beater equipment(e.g. MP
►Biomedicals’ FastPrep®-24 Instrument)
► 96-100% ethanol
Contact Person: Mr. Ye