|DNA Extraction Kit:||Plasmid DNA MaxiPrep Kit (Spin-column)||DNA Isolation Kit:||Plasmid DNA MaxiPrep Kit (Spin-column)|
|DNA Purification Kit:||Plasmid DNA MaxiPrep Kit (Spin-column)|
Plasmid DNA MaxiPrep Kit (Spin-column)
Offered in two sizes (5 preps,10preps,DP1001,DP2501,DP2502)
Bioteke’s Plasmid DNA MaxiPrep Kit is designed for the rapid preparation of plasmid DNA from up to 100 mL of Escherichia coli culture. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/μg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.
Bioteke’s Purification Technology
Purification is based on spin column chromatography. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The process involves first pelleting overnight bacteria culture harbouring plasmid DNA . The bacterial pellet is then resuspended using the provided Resuspension Solution, and the bacteria are lysed using the Lysis Solution. Neutralization Solution is then added, causing the genomic DNA and proteins present in the solution to precipitate. The lysate is then clarified through centrifugation, in order to remove the precipitated proteins and genomic DNA from the lysate containing the plasmid DNA. The clarified lysate is then loaded onto a spin-column. Bioteke’s column binds DNA in a manner that depends on ionic concentrations, thus only the plasmid DNA will bind to the column while most of the digested RNA and proteins will be removed in the flowthough or retained on top of the column bed. The bound DNA is then washed with the provided wash buffer in order to remove any remaining impurities. Lastly, the endotoxin-free purified plasmid DNA is eluted with the elution buffer. The purified DNA is of the highest quality and can be used in a number of downstream applications including sequencing, cloning, and transfections.
Isolate up to 1 mg of high copy number plasmid DNA from 100 mL of culture
Endotoxin-free plasmid DNA - levels of ≤ 0.1EU/μg of plasmid DNA
Fast and easy processing using a rapid spin-column format
Isolate high quality plasmid DNA
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. The RNAse vial shouldbestoredat-20°Cuponarrival. OnceRNAsehasbeenaddedtotheResuspension Buffer the solution should be stored at 4oC. All the reagents should remain stable for at least 1 year in their unopened containers.
Precautions and Disclaimers
This kit is designed for research purposes only. It is not intended for human or diagnostic use. Ensure that a suitable lab coat, disposable gloves and protective goggles are worn when working with chemicals.
The Neutralization Solution contains guanidine hydrochloride, and should be handled with care. Guanidine hydrochloride forms highly reactive compounds when combined with bleach, thus care must be taken to properly dispose of any of this solution.
Customer-Supplied Reagents and Equipment
Centrifuge with a swinging bucket rotor capable of 3000 x g
250 mL centrifuge tubes
Centrifuge capable of 14 000 x g
96 - 100% ethanol
Contact Person: Mr. Ye