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Spin Column DNA Extraction Kit , Plasmid DNA MaxiPrep Kit 5 preps / 10preps

Spin Column DNA Extraction Kit , Plasmid DNA MaxiPrep Kit 5 preps / 10preps

    • Spin Column DNA Extraction Kit , Plasmid DNA MaxiPrep Kit 5 preps / 10preps
    • Spin Column DNA Extraction Kit , Plasmid DNA MaxiPrep Kit 5 preps / 10preps
    • Spin Column DNA Extraction Kit , Plasmid DNA MaxiPrep Kit 5 preps / 10preps
  • Spin Column DNA Extraction Kit , Plasmid DNA MaxiPrep Kit 5 preps / 10preps

    Product Details:

    Place of Origin: Beijing&Wuxi,China
    Brand Name: Bioteke
    Certification: CE
    Model Number: Plasmid DNA MaxiPrep Kit (Spin-column)

    Payment & Shipping Terms:

    Minimum Order Quantity: 100 boxes
    Packaging Details: standard carton box package
    Detailed Product Description
    DNA Extraction Kit: Plasmid DNA MaxiPrep Kit (Spin-column) DNA Isolation Kit: Plasmid DNA MaxiPrep Kit (Spin-column)
    DNA Purification Kit: Plasmid DNA MaxiPrep Kit (Spin-column)

    Plasmid DNA MaxiPrep Kit (Spin-column)

     

    Offered in two sizes (5 preps,10preps,DP1001,DP2501,DP2502)

     

    Bioteke’s Plasmid DNA MaxiPrep Kit is designed for the rapid preparation of plasmid DNA from up to 100 mL of Escherichia coli culture. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/μg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.

     

    Bioteke’s Purification Technology

    Purification is based on spin column chromatography. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The process involves first pelleting overnight bacteria culture harbouring plasmid DNA . The bacterial pellet is then resuspended using the provided Resuspension Solution, and the bacteria are lysed using the Lysis Solution. Neutralization Solution is then added, causing the genomic DNA and proteins present in the solution to precipitate. The lysate is then clarified through centrifugation, in order to remove the precipitated proteins and genomic DNA from the lysate containing the plasmid DNA. The clarified lysate is then loaded onto a spin-column. Bioteke’s column binds DNA in a manner that depends on ionic concentrations, thus only the plasmid DNA will bind to the column while most of the digested RNA and proteins will be removed in the flowthough or retained on top of the column bed. The bound DNA is then washed with the provided wash buffer in order to remove any remaining impurities. Lastly, the endotoxin-free purified plasmid DNA is eluted with the elution buffer. The purified DNA is of the highest quality and can be used in a number of downstream applications including sequencing, cloning, and transfections.

     

    Advantages

    • Isolate up to 1 mg of high copy number plasmid DNA from 100 mL of culture

    • Endotoxin-free plasmid DNA - levels of ≤ 0.1EU/μg of plasmid DNA

    • Fast and easy processing using a rapid spin-column format

    • Isolate high quality plasmid DNA

    Storage Conditions and Product Stability
    All solutions should be kept tightly sealed and stored at room temperature. The RNAse vial shouldbestoredat-20°Cuponarrival. OnceRNAsehasbeenaddedtotheResuspension Buffer the solution should be stored at 4oC. All the reagents should remain stable for at least 1 year in their unopened containers.

     

    Precautions and Disclaimers

    This kit is designed for research purposes only. It is not intended for human or diagnostic use. Ensure that a suitable lab coat, disposable gloves and protective goggles are worn when working with chemicals. 

     

    The Neutralization Solution contains guanidine hydrochloride, and should be handled with care. Guanidine hydrochloride forms highly reactive compounds when combined with bleach, thus care must be taken to properly dispose of any of this solution.

     

    Customer-Supplied Reagents and Equipment

    • Centrifuge with a swinging bucket rotor capable of 3000 x g

    • 250 mL centrifuge tubes

    • Centrifuge capable of 14 000 x g

    • 96 - 100% ethanol

    Spin Column DNA Extraction Kit , Plasmid DNA MaxiPrep Kit 5 preps / 10preps


     

     

     

     

     

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    Contact Details
    BioTeke Corporation

    Contact Person: Mr. Ye

    Send your inquiry directly to us (0 / 3000)

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