|DNA Extraction Kit:||FOOD DNA Isolation Kit||DNA Isolation Kit:||FOOD DNA Isolation Kit|
|DNA Purification Kit:||FOOD DNA Isolation Kit|
FOOD DNA Isolation Kit
Offered in three sizes (50 preps,100preps, 200preps),DP1921,DP1922&DP1923
Bioteke’s Food DNA Isolation Kit provides a rapid method for the isolation and purification of total DNA from a wide range of food samples originating from animals or plants. Bioteke’s Food DNA Isolation Kit is designed for identification of GMO-DNA or animal components in food and feed. Furthermore, the kit also provides a convenient method for the detection of food-related pathogens which may contaminate food sources. Total DNA can be purified from fresh, frozen or preserved food samples using this kit. The purified DNA is of the highest integrity, and can be used in a number of downstream applications including PCR based detection and sequencing.
Bioteke’s Purification Technology
Purification is based on spin column chromatography. The DNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process first involves grinding the food sample in a mortar with liquid nitrogen (or alternative homogenization equipment). CTAB-based Lysis Buffer L and Proteinase K are then added, followed by a short incubation at 55°C. Next, Binding Buffer I is added to the lysate followed by another short incubation on ice. The lysate is then spun in order to remove any debris. Ethanol is added to the clarified lysate, and the solution is loaded onto a spin column. Bioteke’s column binds nucleic acids in a manner that depends on ionic concentrations, thus only the DNA will bind to the column while most of the RNA and proteins are removed in the flowthrough. The bound DNA is then washed with the provided Buffer SK and Wash Solution A in order to remove any remaining impurities, and the purified total DNA is eluted with the Elution Buffer B. The purified DNA is of the highest integrity, and can be used in a number of downstream applications.
Fast and easy processing using a rapid spin-column format
No hazardous chemicals required (e.g. phenol or chloroform)
Wide compatibility with a variety of food products for GMO-DNA isolation
Universal protocol for food related pathogen DNA isolation (Gram positive and Gram
Storage Conditions and Product Stability
The kit contains ready-to-use Proteinase K which is dissolved in a specially prepared storage buffer. The Proteinase K should be stored at room temperature or 4°C. All other solutions should be kept tightly sealed and stored at room temperature.
Precautions and Disclaimers
This kit is designed for research purposes only. This product is not intended for the diagnosis, prevention, or treatment of a disease. Ensure that a suitable lab coat, disposable gloves and protective goggles are worn when working with chemicals.
Buffer SK contains guanidinium salts and should be handled with care. Guanidinium salts forms highly reactive compounds when combined with bleach, thus care must be taken to properly dispose of any of these solutions.
Customer-Supplied Reagents and Equipment
You must have the following in order to use the Food DNA Isolation Kit:
96-100 % ethanol
70 % ethanol
Liquid nitrogen or any mechanical homogenizer
Contact Person: Mr. Ye