|RNA Purification:||Stool Total RNA Purification Kits||RNA Isolation:||Stool Total RNA Purification Kits|
|RNA Extraction:||Stool Total RNA Purification Kits|
Stool Total RNA Purification Kits
Offered in two sizes (50pres,100preps), RP8001&RP8002
Bioteke’s Stool Total RNA Purification Kit provides a convenient and rapid method to purify total RNA from small amounts of stool samples. All types of stool samples can be processed with this kit, including animal fecal samples and manure. The kit removes all traces of humic acid using the provided Bead Tubes and a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the RNA. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA and small interfering RNA. The protocol does not rely on the use of phenol or chloroform, thereby providing a user friendly procedure and allowing high-throughput analysis on the lab bench. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR and reverse transcription PCR for gene expression analysis.
Bioteke’s Purification Technology
Purification is based on spin column chromatography using Bioteke’s proprietary resin as the separation matrix. The process involves first adding the stool sample and Lysis Buffer C to a provided Bead Tube, and the tube is vortexed for 5 minutes in order to efficiently and rapidly homogenize the sample and extract the RNA. The sample is then centrifuged, and the supernatant is transferred to a RNAse-free microcentrifuge tube. An equal volume of ethanol is added to the lysate and the solution is loaded onto a spin-column. Bioteke’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. An optional on-column DNase treatment can be performed at this point also to remove any residual DNA. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Buffer E. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.
No phenol or chloroform extractions
Fast and easy processing (less than 30 minutes for purification)
Isolate high quality total RNA from a variety of stool samples
Process all types of feces from humans and animals
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These reagents should remain stable for at least 1 year in their unopened containers.
Precautions and Disclaimers
This kit is designed for research purposes only. It is not intended for human or diagnostic use. Ensure that a suitable lab coat, disposable gloves and protective goggles are worn when working with chemicals.
Customer-Supplied Reagents and Equipment
You must have the following in order to use Norgen’s Stool Total RNA Purification Kit:
RNAse-free microcentrifuge tubes
Flat bed vortex or bead beater equipment
Customer-Supplied Reagents and Equipment
You must have the following in order to use the Total RNA Purification Kit:
For All Protocols
96 - 100% ethanol
For Animal Cell Protocol
For Animal Tissue Protocol
Mortar and pestle
Working with RNA
RNases are very stable and robust enzymes that degrade RNA. Autoclaving solutions and glassware is not always sufficient to actively remove these enzymes. The first step when preparing to work with RNA is to create an RNase-free environment. The following precautions are recommended as your best defense against these enzymes.
The RNA area should be located away from microbiological work stations
Clean, disposable gloves should be worn at all times when handling reagents, samples,
pipettes, disposable tubes, etc. It is recommended that gloves are changed frequently to
There should be designated solutions, tips, tubes, lab coats, pipettes, etc. for RNA only
All RNA solutions should be prepared using at least 0.05% DEPC-treated autoclaved
water or molecular biology grade nuclease-free water
Clean all surfaces with commercially available RNase decontamination solutions
When working with purified RNA samples, ensure that they remain on ice during
Contact Person: Mr. Ye